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We then performed metagenomic analysis on precisely the same samples to locate the existence of reads from pks+ genes. As demonstrated in Fig. 3, the pks+ genes can be detected while in the sequencing details within the samples (either cancers or standard) in 19 people away from thirty, thus validating the existence of your mutational and indel signatures (see also Supplementary Figs. six and 7). Notably, whereas the presence of pks+ genes in the sample signifies the existence on the genotoxic E. coli strands, the mutational signature is the result of primarily previous exposure, for this reason it could be accumulated in mobile genomes before without the need of pks+ becoming present at this time of sampling.
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The y-axis is the reads counts from E.coli. The crimson/grey shade of the bars denotes the presence/absence of clb genes since the clb genes represent pks genomic island and encoding colibactin.
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